Duodenum Exclusion Alone Is Sufficient to Reduce Fasting Blood Glucose in Non-Obese Diabetic Goto-Kakizaki Rats

To the editor, In addition to reducing weight, bariatric surgery could improve glucose metabolism in patients with type 2 diabetes mellitus [1, 2]. Such actions have mainly been explained by the foregut and the hindgut hypotheses. The foregut hypothesis claims that bariatric surgery removes endogenous substances that cause disturbance in glucose metabolism [3]. The hindgut hypothesis postulates that the glucose-lowering action is mainly the result of the expedited food delivery to the hindgut [4]. However, effects of gastric bypass surgery on glucose metabolism and the relative contribution of the foregut vs. hindgut hypothesis vary considerably based on the type of the surgery. Another major confounding factor is the reflux of gastrointestinal (GI) content into the duodenum [5, 6]. In this preliminary rat study, we examined whether duodenum exclusion alone is sufficient to improve glucose metabolism in a rat model of diabetes. Briefly, the intestine of nonobese diabetic Goto-Kakizaki (GK) rats was transected immediately below the ligament of Treitz. A 3-mm silicon tube was used to connect the duodenum with jejunum and fixed in the wall of jejunum (Fig. 1a). Short-term mortality (<1 week) in this pilot experiment was 100 % (n=55; Table 1). We therefore modified the procedure bywrapping a shorter silicon tube in the duodenum and ligation of the distal end of the duodenum (n=20; Fig. 1b) and achieved a 90 % survival (18/20). Such a modified surgery resulted in a significant reduction of fasting plasma glucose in the following weeks (Fig. 2).